Post-Embedding/On-Section Immunocytochemistry

Cauda equina syndrome post-caesarean section.

Cauda equina syndrome is rarely associated with pregnancy, with few cases reported in the literature. The majority of cases describe antenatal presentations, with only one case manifesting post-partum, three weeks after a normal vaginal delivery. We outline a case of cauda equina syndrome following caesarean section in a patient with known lumbar disc disease, and discuss the contribution of th...

Tissue preparation for immunocytochemistry.

AIMS To investigate the effect of tissue preparation on immunostaining and to establish whether there is a standard tissue preparation schedule that allows optimal demonstration of all antigens. METHODS Blocks of tonsil were subjected to variations to a standard fixation, processing, and section preparation schedule. The sections were stained with five antibodies-L26 (CD20), UCHL1 (CD45RO), C...

Immunocytochemistry: human neural stem cells.

Immunocytochemistry is a very powerful and fairly straightforward method for determining the presence, subcellular localization, and relative abundance of an antigen of interest, most commonly a protein, in cultured cells. This protocol presents an easy-to-follow series of steps that will enable researchers to conserve primary and secondary antibodies while getting high quality, reproducible qu...

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Use of Antibodies in Immunocytochemistry

Immunostaining can be used to pinpont the subcellular localization of a protein antigen, to follow its changing position as cells respond to stumuli, or to compare its position to other proteins in the same cell. Using these methods one can follow an antigen’s distribution during development, mark the location of a particular cell type in a multicellular in vivo setting, or determine the presen...